The Role of Gibberellic Acid in Plant Growth

The Role of Gibberellic Acid in Plant Growth Aim: To study the effect of gibberellic acid application on plant growth. To do this you will set up control and treatment plants. Control plants will be sprayed with water and treatment plants with gibberellic acid (GA) Plants: Your group will use two pots each containing a seedling of Pisum sativum variety William Massey, which is a dwarf variety. The dwarf plants are deficient in gibberellic acid (GA) due to mutation of a gene in the pathway for biosynthesis of GA. Procedure 1. As much as possible, your two plants should match for height. 2. The plant in the square pot will be the control (C) and the plant in the round pot the treatment 3. Label each plant and pot with the appropriate information. Use a pencil for writing labels (pencil does not run/wash off). Using stickers, label the pots with your prac day & time and the seat no. for one member of your group. Using jeweler’s tags label the plants either C or T, depending on pot shape 4. Carefully measure internode length in millimetres. The first internode is the distance between the first node (where the cotyledons were attached) and the second node where the membranous scale leaves are attached. The second internode is the distance between the second node (where the membranous scale leaves are attached) and the third node where the first trifoliate leaf is attached. You may need to use a Vernier caliper if internodes are small. See Appendix I for how to use Vernier calipers. Note, there should be three to four internodes 5. Record the measurements for each plant’s internode length separately in Table P1.1 (next page) under Day 0. If you do not have measurements (i.e. for internodes not yet present) put zero

 

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