Blood Typing Procedure

B. Blood Typing Procedure Procedure: 1. Label each blood typing slide: Slide 1: Mr. Smith / Slide 2: Mr. Jones / Slide 3: Mr. Green / Slide 4: Ms. Brown 2. Place three to four drops of Mr. Smith’s blood in each of the A, B, and Rh wells of Slide$$1.$$3. Place three to four drops of Mr. Jones’s blood in each of the A, B, and Rh wells of Slide 2 . 4. Place three to four drops of Mr. Green’s blood in each of the A, B, and Rh wells of Slide 3 .

5. Place three to four drops of Ms. Brown’s blood in each of the A, B, and Rh wells of Slide$$4.$$6. Place three to four drops of simulated anti-A serum in each$$A$$well on the four slides.

7. Place three to four drops of simulated anti-B serum in each B well on the four slides. 8. Place three to four drops of simulated anti-Rh serum in each$$\mathrm{Rh}$$well on the four slides. 9. Obtain three toothpicks per blood typing slide. Stir each well with a separate clean toothpick for 30 seconds.

To avoid splattering the simulated blood, do not press too hard on the typing tray. 10. Observe each slide and record your observations in the following table. 11. Clean up: WARD’S Simulated Blood is non-biological and nontoxic and may be flushed down the drain.

Check Your Understanding 1. Antigens in the blood are located while antibodies are located

2. What is the adverse reaction that occurs in incompatible (mismatched) blood transfusion? What is the result of this reaction?

3. Which blood type is considered a universal recipient and why?

4. What is the difference between agglutination and coagulation?

5. A woman with severe bleeding was admitted to the emergency room and she is in need of blood transfusion. Her blood type was determined to be$$\mathrm{AB}$$-. Which of the following blood types can she receive?$$O+1\mathrm{A}+1\mathrm{B}+1\mathrm{AB}+1\mathrm{O}-1\mathrm{A}-\mathrm{B}-1\mathrm{AB}-$$