Development department of the drug company

You will help the development department of the drug company Bloodpharmacy to produce 20mg of the protein Tencamirat. Tencamirat is a relatively small (~ 7kDa) protein that is found naturally in relatively low concentrations in the blood. Tencamirate needs to be glycosylated to be functional.

A) Suggest three different possible sources/ways obtain Tencamirat. Name its advantages and limitations.

b) Descriptions of how to prepare samples to purify by chromatography for the respective source material/source.