Which of these series of cloning events represents the best way to clone a gene into a lambda phage?

Which of these series of cloning events represents the best way to clone a gene into a lambda phage?

  1. Partial genomic DNA digestion; ligation into replacement lambda vector; packaging and host infection; hybridization screening; recombinant DNA purification and restriction digestion.
  2. Packaging and host infection; ligation into replacement lambda vector; recombinant DNA purification and restriction digestion; hybridization screening; partial genomic DNA digestion.
  3. Hybridization screening; ligation into replacement lambda vector; packaging and host infection; recombinant DNA purification and restriction digestion; partial genomic DNA digestion.
  4. None of the above is correct

0 replies

Leave a Reply

Want to join the discussion?
Feel free to contribute!

Leave a Reply

Your email address will not be published. Required fields are marked *