Determine the concentration of total protein in each of the RIPA cell lysate samples for the control (C2C12 myoblasts, C) and the treated (C2C12 myotubes, T) cells

Determine the concentration of total protein in each of the RIPA cell lysate samples for the control (C2C12 myoblasts, C) and the treated (C2C12 myotubes, T) cells. Show all calculations and include all units where necessary.

b-Determine the amount of sample volume required to load 20μg of protein for each sample using the concentrations determined in letter a.

Show all calculations and include all units where necessary.

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