List three possible sources of error that may have attributed to the deviation from the theoretical chemotaxis indices for the positive and negative control

List three possible sources of error that may have attributed to the deviation from the theoretical chemotaxis indices for the positive and negative control.

1.) a possible source of error for the negative control is not a high enough concentration of DA was placed on one side. preventing the C. Elegans from detecting the stimulants.

2.) a possible source of error for the positive control is that not enough dsFlNA was introduced into the c.Elegans. this would cause a few C. Elegans to containing functional chemotaxis proteins, allowing them to detect the stimulats.

3.) a possible source of error for the negative control is that the assays were not cleaned properly. the few remaining chemical on the 0 side may attracts C. Elegans, skewing the results.